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2Hx-2细胞

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产品名称: 2Hx-2细胞
产品型号: 2Hx-2
产品展商: HZbscience
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简单介绍

2Hx-2细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。2Hx-2细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。


2Hx-2细胞  的详细介绍

2Hx-2细胞

数量: 大量

是否是肿瘤细胞: 0

物种来源: 小鼠

生长状态: 悬浮生长

细胞形态: **样

**类型: IgG2a

ATCC Number: HB-8117™

运输方式: 冻存运输

Designations: 2Hx-2

Depositors: Massachusetts Institute of Technology

2Hx-2细胞Isotype: IgG2a

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against adenovirus group specific antigen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Comments: Animals were immunized with Hela cells that were infected with adenovirus 2.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

2Hx-2细胞The antibody reacts with adenoviruses of human subgroups A through E as well as with simian, bovine, canine and swine adenoviruses.

It does not react with avian adenoviruses.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. 2Hx-2细胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 1197: Cepko CL, et al. Immunoprecipitation with two-dimensional pools as a hybridoma screening technique: Production and characterization of monoclonal antibodies against adenovirus 2 proteins. Virology 110: 385-401, 1981. PubMed: 7222481

3554: Cepko CL, et al. Production and use of monoclonal antibodies against adenoviruses. US Patent 4,487,829 dated Dec 11 1984

22704: Cepko CL, et al. Adenovirus hexon monoclonal antibody that is group specific and potentially useful as a diagnostic reagent. J. Clin. Microbiol. 17: 360-364, 1983. 2Hx-2细胞PubMed: 6339552

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