MDA-MB-175-VII细胞
运输方式： 冻存运输
细胞形态： 上皮样
器官来源： **
组织来源： duct
数量： 大量
ATCC Number： HTB-25™
相关**： 导管癌
细胞类型： 上皮细胞
生长状态： 贴壁生长
是否是肿瘤细胞： 1
物种来源： 人
MDA-MB-175-VII细胞年限： 56 years
Designations: MDA-MB-175-VII
Depositors: R Cailleau
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: loosely adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: mammary gland; breast
Tissue: duct
Disease: ductal carcinoma
Derived from metastatic site: pleural effusion
Cell Type: MDA-MB-175-VII细胞epithelial
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Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 7,8
D13S317: 11,12
D16S539: 11,13
D5S818: 11
D7S820: 11,12
THO1: 7
TPOX: 6,8
vWA: 15,16
Cytogenetic Analysis: MDA-MB-175-VII细胞model number = 84; range = 82 to 89. The cell line is aneuploid female (XX/XXX/XXXX), with chromosome counts in the hypertetraploid to near-tetraploid range. All normal chromosomes are represented by at least one copy, with most chromosomes having three or four copies present per karyotype. Five marker chromosomes are found: del(1)p35), der(7)t(1;7)(q11;q32), unknown, t(2q;?), unknown isochromosome. Several are present in multiple copy. Alterations in the q arm of chromosome N1 are in accordance with the report by M.J. Siciliano, et al., Cancer Res. 39: 919, 1979.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
PGM1, 2
PGM3, 1-2
Age: 56 years
Gender: female
Ethnicity: Black
Comments: Details on derivation of this line are as described for ATCC HTB-23.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. MDA-MB-175-VII细胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 100%
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
recommended serum:ATCC 30-2020
purified DNA:ATCC HTB-25D
References: 22182: Cruciger Q, et al.MDA-MB-175-VII细胞 Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.
22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779
22532: Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247
22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202
