Caov-4细胞
生长状态： 贴壁生长
细胞形态： 上皮样
ATCC Number： HTB-76™
相关**： 腺癌
器官来源： 卵巢
是否是肿瘤细胞： 1
物种来源： 人
运输方式： 冻存运输
年限： 45 years
数量： 大量
Caov-4细胞Designations: Caov-4
Depositors: J Fogh
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: ovary
Disease: adenocarcinoma
Derived from metastatic site: subserosa of the fallopian tube
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Caov-4细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Cytogenetic Analysis: (P5) hypotriploid and hypertetraploid with abnormalities including breaks, dicentrics, minute chromatin bodies, fragmentations, secondary constrictions and large subtelocentric and submetacentric markers
Isoenzymes: AK-1, 1
ES-D, 2
G6PD, B
GLO-I, 1-2
Me-2, 2
PGM1, 1-2
PGM3, 2
Age: 45 years
Gender: female
Ethnicity: Caucasian
Propagation: Caov-4细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Atmosphere: air, 100%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
Preservation: Freeze medium: Caov-4细胞complete growth medium supplemented with an additional 40% fetal bovine serum and 10% (v/v) DMSO
