SCC-9细胞
数量： 大量
年限： 25 years
是否是肿瘤细胞： 1
物种来源： 人
生长状态： 贴壁生长
ATCC Number： CRL-1629™
相关**： 鳞状细胞癌
运输方式： 冻存运输
器官来源： 舌头
Designations: SCC-9
Depositors: JG Rheinwald
SCC-9细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology:
Source: Organ: tongue
Disease: squamous cell carcinoma
Cellular Products: epidermal keratins; low levels of involucrin
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Applications: transfection host
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 11
D13S317: 9
SCC-9细胞D16S539: 10,11
D5S818: 12
D7S820: 8
THO1: 8,9
TPOX: 9,11
vWA: 17
Age: 25 years
Gender: male
Comments: Growth of SCC-9 is enhanced by using a feeder layer of X-irradiated STO cells (ATCC 56-X).
The cells do not grow well in semi-solid medium.
Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 400 ng/ml hydrocortisone, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: SCC-9细胞Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
feeder layer cells:ATCC 56-X
References: 23039: Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. SCC-9细胞PubMed: 7214336
