MDA-MB-436细胞
生长状态： 贴壁生长
数量： 大量
ATCC Number： HTB-130™
相关**： 腺癌
细胞形态： 其他
年限： 43 years
器官来源： **
是否是肿瘤细胞： 1
物种来源： 人
运输方式： 冻存运输
Designations: MDA-MB-436
MDA-MB-436细胞Depositors: R Cailleau
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: pleomorphic with multinucleated component cells
Source: Organ: mammary gland; breast
Disease: adenocarcinoma
Derived from metastatic site: pleural effusion
Cellular Products: tubulin; actin
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Tumorigenic: No
DNA Profile (STR): MDA-MB-436细胞Amelogenin: X
CSF1PO: 12
D13S317: 10
D16S539: 9,11
D5S818: 13
D7S820: 10
THO1: 9.3
TPOX: 8
vWA: 14,20
Cytogenetic Analysis: modal number = 45
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1
Age: 43 years
Gender: female
Ethnicity: MDA-MB-436细胞Caucasian
Comments: The line is pleomorphic and most cells react intensely with anti tubulin antibody as demonstrated by indirect immunofluorescence staining.
Propagation: ATCC complete growth medium: Leibovitz's L-15 medium with 10 mcg/ml insulin, 16 mcg/ml glutathione, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Atmosphere: air, 100%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Subcultures are prepared by scraping. Remove spent medium, add 3 to 5 ml of fresh medium, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Preservation: Culture medium, 95%; DMSO, 5%
References: 1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337
22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779
22656: Cailleau R, et al. MDA-MB-436细胞Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202
23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903
