H1581细胞
生长状态： 混合型生长
ATCC Number： CRL-5878™
相关**： 非小细胞肺癌
细胞形态： 上皮样
细胞类型： 其他细胞类型
是否是肿瘤细胞： 1
物种来源： 人
年限： stage 4
器官来源： 肺
运输方式： 冻存运输
数量： 大量
Designations: NCI-H1581 [H1581]
Depositors: AF Gazdar, JD Minna
H1581细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: mixed, adherent and suspension
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: lung
Tumor Stage: stage 4
Disease: non-small cell lung cancer
Cell Type: large cell;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. H1581细胞They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.
Age: 44 years
Gender: male
Ethnicity: Caucasian
Comments: The line was established in January 1987.
The patient was a smoker.
75 pack years.
Propagation: ATCC complete growth medium: ACL-4 medium (serum-free)
The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
0.02 mg/ml insulin
0.01 mg/ml transferrin
25 nM sodium selenite (final conc.)
50 nM Hydrocortisone (final conc.)
1 ng/ml Epidermal Growth Factor (do not filter)
H1581细胞0.01 mM ethanolamine (final conc.)
0.01 mM phosphorylethanolamine (final conc.)
100 pM triiodothyronine (final conc.)
0.5% (w/v) bovine serum albumin (final conc.)
0.5 mM sodium pyruvate (final conc.)
extra 2mM L-glutamine (for final conc. of 4.5mM)
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium. Cells grow mostly as floating aggregates of round cell clusters with some attached cells. Clusters are alive while single cells have very low viability. Attached cells can be shaken loose.
Preservation: Freeze medium: culture medium, 85%; FBS, 10%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. H1581细胞Biochem. suppl. 24: 1996
