Hs 913T细胞

Hs 913T细胞

生长状态： 贴壁生长

ATCC Number： HTB-152™

相关**： 纤维肉瘤

数量： 大量

运输方式： 冻存运输

细胞形态： 成纤维样

是否是肿瘤细胞： 1

物种来源： 人

年限： 51 years

Designations: Hs 913T

Depositors: RB Owens

Hs 913T细胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Disease: fibrosarcoma

Derived from metastatic site: lung

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 12

D13S317: 11

D16S539: 13

D5S818: 10

Hs 913T细胞D7S820: 10

THO1: 8,9.3

TPOX: 9,11

vWA: 17,18

Cytogenetic Analysis: modal number = 111 to 113; range = 95 to 120; large submetacentric and double minute (DM) markers

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

PGM1, 2

PGM3, 2

Hs 913T细胞Age: 51 years

Gender: male

Ethnicity: Caucasian

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).

Add fresh medium, aspirate and dispense into new flasks.

Subculture every 6 to 8 days.

Preservation: Hs 913T细胞Culture medium, 95%; DMSO, 5%