A-427细胞
是否是肿瘤细胞： 1
物种来源： 人
运输方式： 冻存运输
年限： 52 years
器官来源： 肺
细胞形态： 上皮样
ATCC Number： HTB-53™
相关**： 肿瘤
数量： 大量
生长状态： 贴壁生长
Designations: A-427
A-427细胞Depositors: DJ Giard
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: lung
Disease: carcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,12
A-427细胞D13S317: 11,12
D16S539: 11,13
D5S818: 12
D7S820: 8,12
THO1: 9
TPOX: 8,11
vWA: 17
Cytogenetic Analysis: at passage 60, hypotriploid to hypertriploid with abnormalities including dicentrics, minutes and large subtelocentric marker
Isoenzymes: AK-1, 2
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 1-2
PGM3, 1
Age: 52 years
Gender: male
Ethnicity: Caucasian
Comments: A-427细胞The A-427 line was derived by D.J. Giard, as indicated in the description for ATCC HTB-41.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Interval: every 6 to 8 days
Subcultivation Ratio: 1:2 to 1:6
Medium Renewal: Twice per week
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
23218: Giard DJ, et al. A-427细胞In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758
