SW 1271细胞
细胞形态： 上皮样
数量： 大量
年限： 69 years
是否是肿瘤细胞： 1
物种来源： 人
运输方式： 冻存运输
生长状态： 贴壁生长
ATCC Number： CRL-2177™
器官来源： 肺
相关**： 小细胞肺癌
Designations: SW 1271 [SW-1271, SW1271]
SW 1271细胞Depositors: W McCombs
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: lung
Disease: carcinoma; small cell lung cancer
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1976
Antigen Expression: Blood Type A; Rh +
DNA Profile (STR): SW 1271细胞Amelogenin: X
CSF1PO: 11
D13S317: 13
D16S539: 11,12
D5S818: 12
D7S820: 9,12
THO1: 7,8
TPOX: 8
vWA: 17,20
Age: 69 years
Gender: male
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Temperature: 37.0°C
Subculturing: SW 1271细胞Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53% mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of cell suspension to new culture vessels.
Place culture vessels in incubators at 37C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: SW 1271细胞liquid nitrogen vapor phase
