Hs 445细胞
器官来源： **结
ATCC Number： HTB-146™
运输方式： 冻存运输
相关**： **瘤
年限： 56 years
是否是肿瘤细胞： 1
物种来源： 人
细胞形态： **样
生长状态： 悬浮生长
数量： 大量
Designations: Hs 445
Hs 445细胞Depositors: RB Owens
Biosafety Level: 2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Organ: lymph node
Disease: Hodgkin's disease; Hodgkin's lymphoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,10.3
D13S317: 11,13
Hs 445细胞D16S539: 9,11
D5S818: 9,12
D7S820: 9,10
THO1: 6,7
TPOX: 8
vWA: 14,17
Isoenzymes: ES-D, 1
G6PD, B
GLO-I, 1-2
PGM1, 1
Age: 56 years
Gender: female
Ethnicity: Caucasian
Comments: Hs 445 was derived in 1970 from an intra-abdominal lymph node of a patient with stage 1 Hodgkin's disease.
By electron microscopy, herpesvirus-like particles have been seen.
0.3% to 0.5% of the cells are positive for EBV early antigen and virion antigen by immunofluorescence.
Propagation: Hs 445细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 4 X 10 exp5 cells/ml and maintain at between 4 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22287: Arnstein P, et al. Propagation of human tumors in antithymocyte serum-treated mice. J. Natl. Cancer Inst. 52: 71-84, 1974. PubMed: 4544026
22550: Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Hs 445细胞Cancer Inst. 65: 715-718, 1980. PubMed: 6932523
