H9/HTLV-IIIB细胞
生长状态： 悬浮生长
数量： 大量
ATCC Number： CRL-8543™
细胞类型： T**细胞
是否是肿瘤细胞： 1
物种来源： 人
运输方式： 冻存运输
细胞形态： **样
Designations: H9/HTLV-IIIB
H9/HTLV-IIIB细胞Depositors: The United States of America
Biosafety Level: 2 [Cells Contain RETROVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Cell Type: T lymphocyte;
Cellular Products: Human T cell leukemia virus III (HTLV III); Human Immunodeficiency virus (HIV)
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Comments: H9/HTLV-IIIB细胞The cells produce HTLV III.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
H9/HTLV-IIIB细胞Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 3536: Popovic M, et al. Serological detection of antibodies to HTLV-III in sera of patients with AIDS and pre-AIDS conditions. US Patent 4,520,113 dated May 28 1985
22938: Chang SY, et al. The origin of HIV-1 isolate HTLV-IIIB. Nature 363: 466-469, 1993. PubMed: 8502298
32754: Trkola A, et al. Human momoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1. J. Virol. 70: 1100-1108, 1996. H9/HTLV-IIIB细胞PubMed: 8551569
