HLF-a细胞

HLF-a细胞

数量： 大量

年限： 54 years

ATCC Number： CCL-199™

运输方式： 冻存运输

相关**： 上皮细胞癌

是否是肿瘤细胞： 1

物种来源： 人

器官来源： 肺

生长状态： 贴壁生长

细胞形态： 成纤维样

HLF-a细胞Designations: HLF-a

Depositors: GD Stoner

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: lung

Disease: epidermoid carcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. HLF-a细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: February, 1976

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11

D13S317: 11,12

D16S539: 12,13

D5S818: 8,12

D7S820: 9,12

THO1: 6,9.3

TPOX: 6,8

vWA: 15

Cytogenetic Analysis: human female; diploid; stable

Isoenzymes: G6PD, B

Age: 54 years

HLF-a细胞Gender: female

Ethnicity: Black

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: culture medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: HLF-a细胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020