SJRH30细胞
是否是肿瘤细胞： 1
物种来源： 人
年限： 17 years
运输方式： 冻存运输
数量： 大量
组织来源： muscle
细胞形态： 成纤维样
生长状态： 贴壁生长
ATCC Number： CRL-2061™
相关**： 横纹肌肉瘤
SJRH30细胞Designations: SJCRH30 [RC13, RMS 13, SJRH30]
Depositors: DN Shapiro
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Tissue: muscle
Disease: rhabdomyosarcoma
Derived from metastatic site: bone marrow
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Oncogene: SJRH30细胞gli + (amplified 30 fold), N-myc + (not amplified)
DNA Profile (STR): D5S818: 12, 13
D13S317: 11
D7S820: 10
D16S539: 12
THO1: 9, 9.3
TPOX: 8, 11
vWA: 17, 18
CSF1PO: 10, 11
Amelogenin: XY
Age: 17 years
Gender: male
Ethnicity: Caucasian
Comments: SJRH30细胞The line was established from cells from the bone marrow of a child with rhabdomyosarcoma.
The cells show ultrastructural elements of primitive skeletal muscle differentiation.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22379: Oliner JD, et al. Amplification of a gene encoding a p53-associated protein in human sarcomas. Nature 358: 80-83, 1992. PubMed: 1614537
23076: Roberts WM, et al. Amplification of the gli gene in childhood sarcomas. Cancer Res. 49: 5407-5413, 1989. PubMed: 2766305
23518: Douglass EC, et al. SJRH30细胞A specific chromosomal abnormality in rhabdomyosarcoma [published erratum appears in Cytogenet Cell Genet 1988;47(4):following 232]. Cytogenet. Cell Genet. 45: 148-155, 1987. PubMed: 3691179
