HMCB细胞
运输方式： 冻存运输
ATCC Number： CRL-9607™
器官来源： 皮肤
相关**： 黑色素瘤
细胞形态： 上皮样
细胞类型： 上皮细胞
是否是肿瘤细胞： 1
物种来源： 人
生长状态： 贴壁生长
数量： 大量
Designations: HMCB [ Human Melanoma Cell Bowes]
Depositors: Genentech, Inc.
HMCB细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: skin
Disease: melanoma
Cell Type: epithelial
Cellular Products: human tissue plasminogen activator
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Applications: transfection host (Roche Transfection Reagents)
Comments: HMCB细胞The cells originally tested positive for mycoplasma.
After treatment with ciprofloxacin, the cells have remained negative for mycoplasma for 60 days.
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) with non-essential amino acids, 10 mM HEPES and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
HMCB细胞Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Two to three times weekly
Preservation: Freeze medium: culture medium 95%; DMSO 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101
Cell culture tested DMSO:HMCB细胞ATCC 4-X
