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RPMI 6666细胞

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产品名称: RPMI 6666细胞
产品型号: RPMI 6666
产品展商: HZbscience
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简单介绍

RPMI 6666细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。RPMI 6666细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。


RPMI 6666细胞  的详细介绍

RPMI 6666细胞

细胞形态: **样

运输方式: 冻存运输

年限: 29 years

生长状态: 悬浮生长

ATCC Number: CCL-113™

器官来源: 外周血

相关**: **瘤

数量: 大量

是否是肿瘤细胞: 1

物种来源: 人

Designations: RPMI 6666

Depositors: GE Moore

RPMI 6666细胞Biosafety Level: 2 [Cell contain Herpesvirus ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: RPMI 6666细胞lymphoblast


Source: Organ: peripheral blood

Disease: Hodgkin's disease; Hodgkin's lymphoma

Cellular Products: immunoglobulin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: HLA A2, A3, B7, B18

Age: 29 years

Gender: male

Ethnicity: Caucasian

Comments: EBNA positive.

Biological Safety CLASS II.

Propagation: RPMI 6666细胞ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20%

Subculturing: Medium Renewal: Add fresh medium (10% to 20% by volume) every 3 to 4 days

Cultures can be maintained by the addition of fresh medium or replacement of medium. Establish new cultures at 5 X 10 exp5 viable cells/ml.

References: 22317: Moore GE, et al. Leukocyte cultures of patients with leukemia and lymphomas. N.Y. State J. Med. 66: 2757-2764, 1966. RPMI 6666细胞PubMed: 5232150

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