1A2细胞
细胞类型： 其他细胞类型
生长状态： 悬浮生长
数量： 大量
是否是肿瘤细胞： 1
物种来源： 人
细胞形态： **样
ATCC Number： CRL-8119™
运输方式： 冻存运输
Designations: 1A2
Depositors: Genetic Systems Corp.
1A2细胞Biosafety Level: 2 [CELLS CONTAIN HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Cell Type: lymphoblast B lymphocyte; Epstein-Barr virus (EBV) transformed
Cellular Products: Epstein-Barr virus (EBV)
Permits/Forms: 1A2细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: This line was derived from the GM1500 lymphoma line.
The cells are resistant to 6-thioguanine.
The cells can be used as a source of infectious EBV, and can be used as driving the cell in"cell driven" EBV transformation of human B cells.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 1 mM sodium pyruvate and 2 mM L-glutamine, 85%; fetal bovine serum, 15%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: 1A2细胞Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 2 X 10(5) and 1 X 10(6) cells/ml.
Interval: every 3 days
Medium Renewal: Every 3 days
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 24 hrs (when cells are in logarithmic growth)
References: 3532: Lostrom ME. Cell-driven viral transfer in eukaryotes. US Patent 4,464,465 dated Aug 7 1984
23264: Croce CM, et al. 1A2细胞Chromosomal location of the genes for human immunoglobulin heavy chains. Proc. Natl. Acad. Sci. USA 76: 3416-3419, 1979. PubMed: 114999
