GA-10 (Clone 4)细胞
年限： 41 years
生长状态： 悬浮生长
运输方式： 冻存运输
细胞形态： **样
数量： 大量
ATCC Number： CRL-2393™
相关**： **瘤
细胞类型： B**细胞
是否是肿瘤细胞： 1
物种来源： 人
Designations: GA-10 (Clone 4)
Depositors: AP Rapoport
GA-10 (Clone 4)细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens deposited as human
Morphology: lymphoblast
Source: Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
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Antigen Expression: CD77 + (90%)
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11
GA-10 (Clone 4)细胞D7S820: 8,10
THO1: 9,9.3
TPOX: 8,11
vWA: 16,20
Age: 41 years
Gender: male
Ethnicity: Caucasian, White
Comments: GA-10 (Clone 4) is a subclone of the GA-10 (ATCC CRL-2392) cell line derived by culturing the parental line on a feeder layer.
GA-10 was derived in 1996 from lymphoma cells circulating in the peripheral blood of a patient with a clinically aggressive, chemo-refractory Burkitt's Lymphoma.
The patient had been treated unsuccessfully with cyclophosphamide, hydroxydaunomycin, vincristine, and prednisone (CHOP chemotherapy).
This clone of cells also appears to express high levels of CD77 (approximately 90%); CD77 is also known as Burkitt's lymphoma antigen (BLA).
GA-10 (Clone-4) cells exhibit sensitivity to Shiga Toxin (Stx) in toxicity studies.
Propagation: GA-10 (Clone 4)细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derived from same cell line:ATCC CRL-2392
derived from same cell line:GA-10 (Clone 4)细胞ATCC CRL-2394
