143B PML BK TK细胞

143B PML BK TK细胞

年限： 13 year old

运输方式： 冻存运输

器官来源： 骨

ATCC Number： CRL-8304™

相关**： 骨肉瘤

是否是肿瘤细胞： 1

物种来源： 人

细胞形态： 成纤维样

数量： 大量

生长状态： 贴壁生长

143B PML BK TK细胞Designations: 143B PML BK TK

Depositors: Wistar Institute

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: bone

Disease: osteosarcoma

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DNA Profile (STR): 143B PML BK TK细胞Amelogenin: X

CSF1PO: 12

D13S317: 12

D16S539: 10,13

D5S818: 13

D7S820: 11,12

THO1: 6

TPOX: 11

vWA: 18

Age: 13 year old

Gender: female

Ethnicity: Caucasian

Comments: Contains a thymidine kinase (TK+) plasmid (PMBKTK).

143B PML BK TK细胞Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with HAT (0.1 mM hypoxanthine, 400 nM aminopterin, 0.16 mM thymidine), 90%; fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Related Products: plasmid in bacteria:ATCC 39369

parental cell line:143B PML BK TK细胞ATCC CRL-8303