G-361细胞

G-361细胞

数量： 大量

器官来源： 皮肤

是否是肿瘤细胞： 1

物种来源： 人

ATCC Number： CRL-1424™

相关**： 恶性黑色素瘤

细胞形态： 上皮样

年限： 31 years *****

运输方式： 冻存运输

Designations: G-361

Depositors: PT Peebles

G-361细胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: skin

Disease: malignant melanoma

Cellular Products: melanin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X

CSF1PO: 10

D13S317: 11

G-361细胞D16S539: 15

D5S818: 11

D7S820: 8,11

THO1: 7,9

TPOX: 8,10

vWA: 15,17

Isoenzymes: G6PD, B

Age: 31 years *****

Gender: male

Ethnicity: Caucasian

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. G-361细胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin - 0.53 mM EDTA for several minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 24 hours after seeding from a thawed ampule, then 2 to 3 times per week

References: 22209: . . Pediatr. G-361细胞Res. 12: 485, 1978.