JM1细胞
细胞类型： 其他细胞类型
数量： 大量
是否是肿瘤细胞： 1
物种来源： 人
运输方式： 冻存运输
ATCC Number： CRL-10423™
相关**： 其他**
生长状态： 悬浮生长
细胞形态： **样
JM1细胞Designations: JM1
Depositors: Immunex Corporation
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: B cell lymphoma
Cell Type: pre-B lymphoblast;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. JM1细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: HLA-DR; Homo sapiens, expressed
CD19; Homo sapiens, expressed
CD10; Homo sapiens, expressed
DNA Profile (STR): D5S818: 12
D13S317: 11
D7S820: 8, 10
D16S539: 9
vWA: 17
THO1: 6
TPOX: 10, 11
CSF1PO: 12
Amelogenin: X Y
Comments: JM1细胞The JM1 cell line was established from cells taken from a patient with immunoblastic B cell lymphoma - leukemia.The cells are negative for CD7, My 7, My 9 and surface IgM.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
0.05 mM 2-mercaptoethanol
fetal bovine serum to a final concentration of 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Alternatively, cultures may be established by centrifugation. Adherent cells can be dislodged by scraping. Initiate cultures at 2 to 5 x 10(5) viable cells/ml. Subculture when cell density reaches 1 X 10(6) cells/ml.
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: culture medium, 95%; DMSO 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: JM1细胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
Cell culture tested DMSO:ATCC 4-X
References: 91916: Park LS, et al. Heterogeneity in human interleukin-3 receptors. A subclass that binds human granulocyte/macrophage colony stimulating factor. J. Biol. Chem. 264: 54205427, 1989. PubMed: 2647717
