C-4I细胞
生长状态： 贴壁生长
ATCC Number： CRL-1594™
相关**： 肿瘤
是否是肿瘤细胞： 1
物种来源： 人
细胞形态： 上皮样
数量： 大量
年限： 41 years
运输方式： 冻存运输
器官来源： 宫颈
Designations: C-4 I
C-4I细胞Depositors: N Auersperg
Biosafety Level: 2 [Cells contain human papilloma virus ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: cervix
Disease: carcinoma
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Tumorigenic: Yes
Isoenzymes: G6PD, B
Age: 41 years
Gender: female
Ethnicity: Caucasian
Comments: C-4I细胞This is one of two lines developed from this patient (see ATCC CRL 1595).
The line contains human papillomavirus type 18 (HPV-18) DNA sequences, and expresses HPV-18 RNA.
Propagation: ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 3 times per week
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting
C-4I细胞Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
References: 22514: Auersperg N, Hawryluk AP. Chromosome observations on three epithelial-cell cultures derived from carcinomas of the human cervix. J. Natl. Cancer Inst. 28: 605-627, 1962. PubMed: 13863218
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
23027: Herz F, et al. Chromosome analysis and alkaline phosphatase of C41, a cell line of human cervical origin distinct from HeLa. Cancer Res. 37: 3209-3213, 1977. PubMed: 560251
23180: Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217
26098: Auersperg N. Histogenetic behavior of tumors. I. Morphologic variation in vitro and in vivo of two related human carcinoma cell lines. J. Natl. Cancer Inst. 43: 151-173, 1969. PubMed: 5796380
26099: Auersperg N. Histogenetic behavior of tumors. II. Roles of cellular and environmental factors in the in vitro growth of carcinoma cells. J. Natl. Cancer Inst. 43: 175-190, 1969. C-4I细胞PubMed: 5796382
26100: Auersperg N. Histogenetic behavior of tumors. 3. Possible relationships to patterns of glycolysis. J. Natl. Cancer Inst. 48: 1589-1596, 1972. PubMed: 5056250
