HT细胞
年限： 70 years
数量： 大量
ATCC Number： CRL-2260™
相关**： 其他**
组织来源： ascites
运输方式： 冻存运输
细胞形态： **样
**类型： kappa
生长状态： 悬浮生长
细胞类型： B**细胞
是否是肿瘤细胞： 1
物种来源： 人
HT细胞Designations: HT
Depositors: M Beckwith
Isotype: kappa
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Tissue: ascites
Disease: diffuse mixed lymphoma
Cell Type: B lymphoblast;
Cellular Products: immunoglobulin; kappa light chain
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Isolation: HT细胞Isolation date: 1983
Antigen Expression: CD19 +; CD20 +; CD21 +; CD22 +; Hle-1 +; HLA DQ +; HLA DR +; CD25 -; T cell receptor (TCR) -
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10
D13S317: 13,14
D16S539: 11,13
D5S818: 11,13
D7S820: 8,10
THO1: 6,7
TPOX: 11
HT细胞vWA: 17,18
Age: 70 years
Gender: male
Ethnicity: Caucasian
Comments: HT is a human B cell lymphoma cell line originated in 1983 by Walter J. Urba and Dan L. Longo.
HT cells express mRNA for both IgM and kappa and express low levels of kappa on the cell surface.
Exposure of HT cells to protein kinase C activating phorbol esters such as PMA and PdBu induced profound growth inhibition.
HT cells have been reported to be Epstein-Barr virus genome negative.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10(5) viable cells/ml and maintain between 1 X 10(5) and 1 X 10(6).
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: HT细胞Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22555: Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723
