Hs 706.T细胞

Hs 706.T细胞

ATCC Number： CRL-7447™

相关**： 巨细胞肉瘤

年限： 16 years

生长状态： 贴壁生长

数量： 大量

器官来源： 骨

是否是肿瘤细胞： 1

物种来源： 人

运输方式： 冻存运输

Hs 706.T细胞细胞形态： 成纤维样

Designations: Hs 706.T

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: bone

Disease: giant cell sarcoma

Permits/Forms: Hs 706.T细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X

CSF1PO: 12,13

D13S317: 8,13

D16S539: 13

D5S818: 11,12

D7S820: 9,10

THO1: 8,9

TPOX: 8,10

vWA: 16,18

Hs 706.T细胞Age: 16 years

Gender: female

Ethnicity: Caucasian

Comments: Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.

Please see the NBL Repository description.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: Hs 706.T细胞Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase