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Hs 602细胞

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产品名称: Hs 602细胞
产品型号: Hs 602
产品展商: HZbscience
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简单介绍

Hs 602细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。Hs 602细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。


Hs 602细胞  的详细介绍

Hs 602细胞

是否是肿瘤细胞: 1

物种来源: 人

ATCC Number: HTB-142™

相关**: **瘤

器官来源: 其他

数量: 大量

生长状态: 悬浮生长

运输方式: 冻存运输

细胞形态: 其他

Hs 602细胞Designations: Hs 602

Depositors: P Arnstein

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: single spherical or small clusters


Source: Organ: cervical lymph node

Disease: lymphoma

Permits/Forms: Hs 602细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Cytogenetic Analysis: no Y chromosome noted

Isoenzymes: ES-D, 1

G6PD, B

GLO-I, 1-2

PGM1, 1-2

Gender: female

Propagation:Hs 602细胞 ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 3 X 10 exp5 cells/ml and maintain at between 4 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22287: Arnstein P, et al. Propagation of human tumors in antithymocyte serum-treated mice. J. Natl. Cancer Inst. 52: 71-84, 1974. PubMed: 4544026

22550: Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Hs 602细胞Cancer Inst. 65: 715-718, 1980. PubMed: 6932523

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