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NCI-H2107细胞

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产品名称: NCI-H2107细胞
产品型号: NCI-H2107
产品展商: HZbscience
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简单介绍

NCI-H2107细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。NCI-H2107细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。


NCI-H2107细胞  的详细介绍

NCI-H2107细胞

器官来源: 肺

年限: stage E

ATCC Number: CRL-5983™

相关**: 小细胞肺癌

运输方式: 瓶装运输

数量: 大量

是否是肿瘤细胞: 1

物种来源: 人

细胞形态: 上皮样

生长状态: 悬浮生长,簇状悬浮

Designations: NCI-H2107 [H2107]

Depositors: AF Gazdar, JD Minna

NCI-H2107细胞Biosafety Level: 1

Shipped: flask

Medium & Serum: See Propagation

Growth Properties: clusters in suspension

Organism: Homo sapiens

Morphology: epithelial


Source: Organ: lung

Tumor Stage: stage E

Disease: carcinoma; small cell lung cancer

Derived from metastatic site: bone marrow

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. NCI-H2107细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

Isolation: Isolation date: December, 1988

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11

D13S317: 12,13

D16S539: 10,13

D5S818: 14

D7S820: 8

THO1: 6,7

TPOX: 10,11

NCI-H2107细胞vWA: 17

Age: 36 years

Gender: male

Ethnicity: Black

Propagation: ATCC complete growth medium: HITES medium supplemented with 5% fetal bovine serum

The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium

0.005 mg/ml Insulin

0.01 mg/ml Transferrin

30nM Sodium selenite (final conc.)

10 nM Hydrocortisone (final conc.)

10 nM beta-estradiol (final conc.)

extra 2mM L-glutamine (for final conc. of 4.5 mM)

5% fetal bovine serum (final conc.)


Temperature: 37.0°C

Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: normal (or near-normal) cell line established from the same patient:ATCC CRL-5966

References: 23570: . NCI-H2107细胞NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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