29SR细胞
生长状态： 悬浮生长
ATCC Number： CRL-2262™
相关**： 其他**
年限： 11 years
数量： 大量
运输方式： 冻存运输
细胞形态： **样
细胞类型： **母细胞
是否是肿瘤细胞： 1
物种来源： 人
Designations: SR
Depositors: M Beckwith
29SR细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: large cell immunoblastic lymphoma
Derived from metastatic site: pleural effusion
Cell Type: lymphoblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: Hle-1 +; HLA DQ +; HLA DR +; CD25 +; CD19 -; CD20 -; CD21 -; CD22 -; T cell receptor (TCR) -
DNA Profile (STR): 29SR细胞Amelogenin: X,Y
CSF1PO: 14,15
D13S317: 11
D16S539: 11,13
D5S818: 11
D7S820: 9,12
THO1: 6,7
TPOX: 8,11
vWA: 17,18
Age: 11 years
Gender: male
Ethnicity: Caucasian
Comments: SR is a human lymphoma cell line originated in 1983 by Walter J. Urba and Dan L. Longo.
29SR细胞SR is of undetermined cellular origin because it expresses no markers unique to B, T, natural killer (NK) or monocyte-lineages.
Exposure of SR cells to protein kinase C activating phorbol esters such as PMA and PdBu do not induce growth inhibition.
SR cells have been reported to be Epstein-Barr virus genome negative.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22555: Beckwith M, et al. 29SR细胞Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723
