K-12 MG1655大肠杆菌克隆菌使用说明：
沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品三个月内通知我司。收到甘油菌后，请及时在固体平板上划线培养，挑取单菌落接种到液体培养基中，震荡培养后使用并保存甘油菌。如需获取其他详细信息请登录我司官网联系QQ客服查询。
基因型：
F- lambda- ilvG- rfb-50 rph-1(NC_000913)
K-12 MG1655大肠杆菌克隆菌基本信息：
抗性：无
培养基：LB
菌株类别：大肠杆菌
培养条件：37℃，有氧，LB
质粒转化：42℃热激
保存方式：20%甘油，-20℃
基本应用：用于基因克隆
备注：
K-12 MG1655大肠杆菌克隆菌菌株简介：
K-12 MG1655是一株K系列的大肠杆菌，经检测无Amp和Kan抗性，可用LB培养基，37℃培养，其代谢类型是异养兼性厌氧型，可用于代谢研究、基因编辑等实验。
The original K-12 wild-type strain from the Stanford collection contained both the F plasmid and phage lambda. MG1655 was constructed by curing that strain using acridine orange and UV.This strain was sequenced by the Blattner laboratory because it approximates wild-type E. coli and "has been maintained as a laboratory strain with minimal genetic manipulation, having only been cured of the temperate bacteriophage lambda and F plasmid by means of ultraviolet light and acridine orange, respectively." (Blattner, et al. 1997). The mutations listed in the genotype are present in most K-12 strains and were probably acquired early in the history of the laboratory strain. A frameshift at the end of rph results in decreased pyrE expression and a mild pyrimidine starvation, such that the strain grows 10 to 15% more slowly in pyrimidine-free medium than in medium containing uracil (Jensen 1993). The ilvG- mutation is a frameshift that knocks out acetohydroxy acid synthase II (Lawther, et al. 1982). The rfb-50 mutation is an IS5 insertion that results in the absence of O-antigen synthesis (Liu and Reeves 1994).
MG1655 was derived and named by Mark Guyer from strain W1485, which was derived in Joshua Lederberg's lab from a stab-culture descendant of the original K-12 isolate. This original E. coli strain K-12 was obtained from a stool sample of a diphtheria patient in Palo Alto, CA in 1922 (Bachmann, B., pp. 2460-2488 in Neidhardt et al.1996, Escherichia coli and Salmonella: Cellular and Molecular Biology, ASM Press).MG1655 grows on LB and M9 minimal medium (+ Glucose + 1ug/ml thiamine).
K-12 MG1655大肠杆菌克隆菌注意事项：
1、为了您的**和健康，请穿实验服并戴一次性手套操作。本产品仅可用于实验室研究，不能用于动物，人体以及作为食品添加剂等用途。
2、使用甘油菌时可不完全融解，在甘油菌表面蘸取少量涂布固体琼脂平板即可，也可完全融解后使用，但随着冻融次数的增加，菌株的活力会逐渐下降。hz1094 木糖赖氨酸去氧胆酸盐琼脂（XLD） 250g 选择性培养基，用于分离志贺氏菌属，也可用于分离沙门氏菌属
yblose Lysine Desoybcholate Medium
hz1095 志贺氏菌增菌肉汤 250g 用于志贺氏菌增菌培养
Shigella Broth
hz1096 WS琼脂 250g 用于沙门氏菌的选择性分离（GB标准）
WS Salmonella Agar
hz1097 氯化镁孔雀绿增菌液 250g 用于沙门氏菌选择性增菌培养(ISO标准)
Rappaport Vassiliadis Medium
hz1098 酚红煌绿琼脂 250g 用于沙门氏菌分离培养（GB标准）
Phenolphthalein Brilliant Green Agar
hz1099 葡萄糖铵培养基 250g 用于志贺氏菌的葡萄糖铵试验（GB标准）
Ammonium Dextrose Medium
hz1100 葡萄糖半固体培养基 250g 用于志贺氏菌的复合生化试验（GB标准）
Dextrose Semisolid Medium
hz1101 动力-吲哚-尿素培养基基础（MIU） 250g 用于**的复合生化试验
Motility Indol Urea Medium Base
hz1102 中国蓝琼脂培养基 250g 用于肠道菌选择性分离培养
China Blue Medium