Bac-to-BacHT Vector Kit 设计用于在大肠杆菌 (E. coli) 中生成杆状病毒载体后，在 Sf9、Sf21 或 High Five 细胞中表达和纯化带组氨酸标签的重组蛋白。 pFastBacHT 载体具有以下特点：
用于表达蛋白质的多角体强启动子
用于简化克隆的三个读码框
用于轻松纯化重组融合蛋白的 N-端 6xHis 标签
用于在蛋白质纯化后去除组氨酸标签的 TEV 蛋白酶切割位点
引用及参考文献
DEF6, a Novel Substrate for the Tec Kinase ITK, Contains a Glutamine-rich Aggregation-prone Region and Forms Cytoplasmic Granules that Co-localize with P-bodies.
Hey F, Czyzewicz N, Jones P, Sablitzky F,
J Biol Chem (2012) 287:31073-31084
Product usage: na
Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin.
Dan C; Kelly A; Bernard O; Minden A;
J Biol Chem (2001) 276:32115-32136
Product usage: pFASTBAC/HT and pFASTBAC-1 vectors were used to transfect with Sf9 cells with Cellfectin.
Sp3 Represses Gene Expression via the Titration of Promoter-specific Transcription Factors.
Kennett Sarah B; Moorefield K Scott; Horowitz Jonathan M;
J Biol Chem (2002) 277:9780-9789
Product usage: Baculovirus stocks encoding Sp1, Sp3, M1, and M2 were prepared using the PCR, appropriate primers, and pCMV4-Sp1/flu, pBSK-Sp3/flu, pCR-M1/flu,
and pCR-M2/flu as substrates. PCR reactions employed pGEX-M2 using Taq polymerase. Amplified cDNAs were subcloned in pFASTBacHTA.
