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pLVX-shRNA1

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产品名称: pLVX-shRNA1
产品型号:
产品展商: HZbscience
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简单介绍

pLVX-shRNA1的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到pLVX-shRNA1后请短暂离心,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。


pLVX-shRNA1  的详细介绍

pLVX-shRNA1载体基本信息

载体名称: pLVX-shRNA1 , pLVX shRNA1
质粒类型: 哺乳动物细胞慢病毒表达载体,shRNA,RNA干扰载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 7758 bp
5' 测序引物及序列: LKO.1 5’:GACTATCATATGCTTACCGT
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄
筛选标记: 嘌呤霉素
备注: 含有组成型人U6启动子的慢病毒shRNA载体
产品目录号: 632177
稳定性: /
组成型: --
病毒/非病毒: 慢病毒

pLVX-shRNA1载体质粒图谱和多克隆位点信息

pLVX-shRNA1载体图谱


pLVX-shRNA1多克隆位点

载体简介

Description

pLVX-shRNA1 is an HIV-1-based, lentiviral expression vector designed to express a small hair-pin RNA (shRNA) for RNA interference (RNAi) studies. Expression of your shRNA is drivenby the RNA Pol III-dependent, human U6 promoter (PU6), located just upstream of the MCS. pLVX-shRNA1 can either be used as a plasmid expression vector and transfected into cells, or it can be packaged into viral particles and transduced into cells. Lentiviral particles derived from the vector allow the expression of shRNAs in virtually any cell type, including primary cells.

pLVX-shRNA1 contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral RNA (1), leading to increased viral titers from packaging cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus (2). Finally, pLVX-shRNA1 also contains a central polypurine tract/central termination sequence element (cPPT/CTS). During target cell infection, thiselement creates a central DNA flap that increases nuclear import of the viral genome, resulting in improved vector integration and more efficient transduction (3).

In addition to lentiviral elements, pLVX-shRNA1 contains a puromycin resistance gene (Puror)under the control of the murine phosphoglycerate kinase promoter (PPGK) for the selection of stable transductants. The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) for propagation and selection in bacteria.

Use
pLVX-shRNA1 is available in the Lenti-X™ shRNA Expression System (Cat. No. 632177). Before it can be transduced into target cells, the vector must be cotransfected into 293T cells with our Lenti-X HTX Packaging System (Cat. Nos. 631247 and 631249) and packaged into viral particles. This packaging system allows the safe production of high titer, infectious, replication-incompetent, VSV-G pseudotyped lentiviral particles that can infect a wide range of cell types, including non-dividing and primary cells (4).

pLVX-shRNA1载体序列

hz-6632R NESG1/CCDC19  鼻咽上皮细胞特异性蛋白1抗体
hz-6633R FOXL2  小睑裂综合征蛋白BPES抗体
hz-6634R XPC  DNA补充修复XPC细胞蛋白抗体
hz-6636R EPHA10  酪氨酸蛋白激酶受体A10抗体
hz-6637R TP53I8/EI24  肿瘤蛋白p53诱导蛋白8
hz-6638R EVC2  膜蛋白EVC2抗体
hz-6639R BMPR1B  骨形态发生蛋白受体1B抗体
hz-6640R Vinculin  粘着斑蛋白抗体
hz-6641R DNA Ligase 1  DNA连接酶1抗体
hz-6642R FOXC1/FREAC3  叉头相关转录因子3抗体
hz-6643R SCYL1BP1  SCYL结合蛋白1抗体
hz-6644R Gephyrin  桥尾蛋白抗体
hz-6645R WNT7A  原癌基因wnt7a蛋白抗体
hz-6646R BLK/MODY11  B**细胞酪氨酸激酶抗体
hz-6647R COG1  COG1蛋白抗体
hz-6648R BAP31  BAP31蛋白抗体
hz-6649R ADORA1/Adenosine A1 Receptor  腺苷A1受体抗体
hz-6650R SDHB  铬细胞瘤患者抑癌基因SDHB抗体
hz-6651R ApoER2  载脂蛋白E2受体抗体
hz-6652R CRMP1  二氢嘧啶酶相关蛋白1抗体
hz-6653R IFI78/MX1  干扰素诱导蛋白P78抗体
hz-6654R ADA/Adenosine deaminase  腺苷脱氨酶抗体