pRI101-ON载体简介：
pRI101 DNA vectors are binary plant transformation vectors intended for foreign gene
expression in plant cells. These agrobacterium-mediated plant transformation vectors carry
the 35S promoter of caμliflower mosaic virus (CaMV) and the 5’ non-coding region (5’-UTR)
of the alcohol dehydrogenase (ADH) gene. The 5’-UTR of ADH functions as a translation
enhancer in plants. There are two types of pRI101 DNA vectors, the pRI101-AN DNA vector,
which carries the 5’-UTR of Arabidopsis ADH (AtADH 5’-UTR), and the pRI101 ON DNA vector,
which carries the 5’-UTR of rice ADH (OsADH 5’-UTR). The pRI101-AN DNA vector is for
dicotyledonous plants such as tobacco or Arabidopsis while the pRI101-ON DNA vector is for
monocotyledonous plants such as rice.
Although the pRI101 DNA vectors are shuttle vectors and replicate autonomously in E. coli and
Rhizobium (Agrobacterium), they are high copy number plasmids because they contain the same
replication origin as pUC-type plasmids (ColE1 ori). These vectors are also stably maintained
in Rhizobium (Agrobacterium) containing the mutant-type replication origin Ri (Ri-ori). The
pRI101 DNA vectors are capable of stably integrating target genes into plant chromosomes
because the vector cloning sites are located closer to the Right Border (RB) of T-DNA than
the selection marker (NPT II) of the plant, so the target gene is not deleted.
pRI101-ON载体特征：
Agrobacterium-mediated plant transformation
Easy and stable integration of dicot and monocot target genes into plant chromosomes
Increased target gene expression via the 5'-UTR translation enhancer region
Vector versatility for dicotyledonous and monoctyledonous plant cells
参考文献：
1. Sugio, T., et al. (2008) J. Bioscience and Bioengineering 105(3):300-302.
2. Nishiguchi, R. et al. (1987) Mol. and Gen. Genet. 206:1-8.
3. Satoh, J. et al. (2004) J. Bioscience and Bioengineering 98(1):1-8.
