pCas-Guide-EF1a-GFP All-in-one vector
Expresses human codon-optimized Cas9, C-terminal Myc-DDK tagged
BamH I and BsmB I can be used for genomic target sequence cloning
Expresses gRNA containing the inserted target sequence and the gRNA scaffold
GFP as a reporter for tracking or sorting transfected cells
CF3 primer can be used to sequence the target sequence after cloning