Product Name	Rabbit anti-EEA1 polyclonal antibody - N-terminal region
Antibody Type	Primary Antibodies
Immunogen	The immunogen for anti-EEA1 antibody: synthetic peptide directed towards the N terminal of human EEA1

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	WBIP
Species Reactivity	BovineDogGuinea PigHorseHumanMousePigRabbitRat
Concentration	1mg/ml
Purification	Affinity purified

Gene Symbol	EEA1
Gene Synonyms	EEA1
Gene Full Name	Early endosome antigen 1
Gene Summary	EEA1 is involved in neuronal synaptic vesicle function and axonal transport and growth. EEA1 may undergo calcium-dependent conformational changes that are required for binding to SNAP-25.
Alternative Names	MST105 ZFYVE2 MSTP105
Molecular Weight(MW)	162kDa
Sequence	1411 amino acids

Application

Rabbit Anti-EEA1 Antibody
Catalog Number:
Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver
Observed Staining: Cytoplasm in hepatocytes, moderate signal, moderate tissue distribution
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 – 2.0 sec
Protocol located in Reviews and Data.
Application

WB Suggested Anti-EEA1 Antibody Titration: 0.06ug/ml
ELISA Titer: 1:312500
Positive Control: Human Placenta
Application

Amount and Sample Type :
500 ug rat brain homogenate
Amount of IP Antibody :
6 ug
Primary Antibody :
EEA1
Primary Antibody Dilution :
1:500
Secondary Antibody :
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution :
1:5000
Gene Name :
EEA1
Submitted by :
Dr. Yuzhi Chen, University of Arkansas for Medical Science

Form	Liquid
Storage Instructions	Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.