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GFP Antibody

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产品名称: GFP Antibody
产品型号:
产品展商: immunoclone
产品文档: 无相关文档

简单介绍

GFP Antibody迅速增长的产品线包括一抗、二抗、**测定及细胞检测试剂盒、蛋白/多肽、激动剂/拮抗剂/活化剂/抑制剂及裂解物等。GFP Antibody严格的质量控制和验证实验包括各种不同的应用：WB、细胞**荧光、**组化、流式细胞术、ELISA、CHIP、IP和peptide array。


GFP Antibody  的详细介绍

GFP AntibodyProduct Profile

Product Name GFP Antibody (FL) AC
Antibody Type Tags Antibodies
Modification Notes The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.

GFP AntibodyKey Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBIPIFELISA

recommended for detection of GFP and GFP mutant fusion proteins by WB, IP, IF and ELISA:
Species Reactivity

HumanMouseRat

Concentration 500 µg/ml, 25% ag
Purification Affinity purified

GFP AntibodyTarget Information

Alternative Names
epitope corresponding to amino acids 1-238 representing full length GFP (green fluorescent protein) of Aequorea victoria origin
Tissue Specificity epitope corresponding to amino acids 1-238 representing full length GFP (green fluorescent protein) of Aequorea victoria origin

GFP AntibodyDatabase Links

GFP AntibodyApplication

  • Application

    Western blot analysis of GFP fusion protein expression in non-transfected COS cells (A) and COS cells transfected with pCruz GFP-Lac Z: (B).

  • Application

    Fluorescence staining of methanol-fixed COS cells transfected with pCruz GFP-Lac Z: , showing GFP-Lac Z localization in the cytoplasm.

Application Notes
recommended for detection of GFP and GFP mutant fusion proteins by WB, IP, IF and ELISA:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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