Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
Tested Applications	ELISAIHC-WIPWB
	*Optimal dilutions/concentrations should be determined by the researcher.: WB:1:500~1:2000 IP:1:100~1:500
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration)
Purification	Affinity purified

Gene Synonyms	6X His tag antibody His antibody 6x His antibody 6x his tag antibody His Tag antibody 6xHis Tag antibody 6 x His antibody More

Application

6X His tag antibody detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 2.5 μg/ml 6x His antibody . Bound Abs were further detected by using HRP-conjugated goat anti-rabbit IgG with HRP substrates.
Application

Sample A: 10 µg of non-transformed E. coli lysate B: 10 µg of non-transformed E. coli lysate, induced C: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced D: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced 12% SDS PAGE diluated at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application

His-tagged protein was immunoprecipitated from transfected cells using 8 ug of 6X His tag antibody . The precipitated His-tagged protein was detected by diluted at 1:500, and used EasyBlot anti-rabbit IgG as secondary reagent.
Application

Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing His-tagged protein (~19 kDa), using 6X His tag antibody. A: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced (10 µg). B: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced (10 µg). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Positive Control	His-tagged recombinant protein
Application Notes	*Optimal dilutions/concentrations should be determined by the researcher.: WB:1:500~1:2000 IP:1:100~1:500

Form	Liquid
Storage Instructions	Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.