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StrepII-tag Antibody

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产品名称: StrepII-tag Antibody
产品型号:
产品展商: immunoclone
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简单介绍

StrepII-tag Antibody迅速增长的产品线包括一抗、二抗、**测定及细胞检测试剂盒、蛋白/多肽、激动剂/拮抗剂/活化剂/抑制剂及裂解物等。StrepII-tag Antibody严格的质量控制和验证实验包括各种不同的应用：WB、细胞**荧光、**组化、流式细胞术、ELISA、CHIP、IP和peptide array。


StrepII-tag Antibody  的详细介绍

StrepII-tag AntibodyProduct Profile

Product Name StrepII-tag Antibody
Antibody Type Tags Antibodies
Product description 
					

						StrepII-tag Antibody: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered to allow it to also placed at the N-terminus of recombinant proteins.1) Schmidt TGM and Skerra A. The random peptide-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Eng.1993; 6:109-22.
					

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Immunogen 
StrepII-tag antibody was raised against the amino acid sequence "asWSHPQFEKga".

StrepII-tag AntibodyKey Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ELISAWB

StrepII-tag antibody can be used for the detection of StrepII-tagged proteins by Western blot at 1 μg/mL.:
Species Reactivity

Tag

Concentration 1mg/ml
Purification Affinity purified

StrepII-tag AntibodyTarget Information

Alternative Names
StrepII-tag Antibody:

Database Links

StrepII-tag AntibodyApplication

  • Application

    Western blot analysis of (A) 5 and (B) 25ng of StrepII-tagged recombinant protein with StrepII antibody at 1 μg/mL.

Application Notes
StrepII-tag antibody can be used for the detection of StrepII-tagged proteins by Western blot at 1 μg/mL.:

StrepII-tag AntibodyAdditional Information

Form Liquid
Storage Instructions StrepII-tag antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer StrepII-tag Antibody is supplied in PBS containing 0.02% sodium azide.

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