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Rb (phospho Ser795) Antibody

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产品名称: Rb (phospho Ser795) Antibody
产品型号:
产品展商: immunoclone
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简单介绍

Rb (phospho Ser795) Antibody迅速增长的产品线包括一抗、二抗、**测定及细胞检测试剂盒、蛋白/多肽、激动剂/拮抗剂/活化剂/抑制剂及裂解物等。Rb (phospho Ser795) Antibody严格的质量控制和验证实验包括各种不同的应用：WB、细胞**荧光、**组化、流式细胞术、ELISA、CHIP、IP和peptide array。


Rb (phospho Ser795) Antibody  的详细介绍

Rb (phospho Ser795) AntibodyProduct Profile

Product Name Rb (phospho Ser795) Antibody
Antibody Type Primary Antibodies
Product description 
					

						Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.1) Fang Sun, Hanjiang Fu, Qin Liu, et al. (2008) Downregulation of CCND1 and CDK6 by miR-34a induces cell cyclearrest. FEBS Letters, 582:1564–1568.
					

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Immunogen 
Rb (Phospho-Ser795) antibody was raised against a peptide sequence around phosphorylation site of serine 795 (P-S-S (p) -P-L) derived from Human Rb.

Rb (phospho Ser795) AntibodyKey Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IFIHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Rb (phospho Ser795) AntibodyTarget Information

Gene Symbol RB1
Alternative Names
RB
pRb
OSRC
pp110
p105-Rb
ASK1
MAPKKK5
MEKK5
P105-RB
PP105
PP110
RB-1
RB1
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Molecular Weight(MW) 110 kDa
Tissue Specificity This antibody detects endogenous level of Rb only when phosphorylated at serine 795.

Rb (phospho Ser795) AntibodyDatabase Links

Entrez Gene 5925
Protein Accession NP_000312.2

Rb (phospho Ser795) AntibodyApplication

  • Application

    Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb (Phospho-Ser795). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Rb (Phospho-Ser795).

  • Application

    Western blot analysis of lysed extracts from K562 cells untreated (Lane 1) or treated with serum (lane 2) using Rb (Phospho-Ser795).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:

Additional Information

Form Liquid
Storage Instructions Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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