产品资料

p70 S6 Kinase Antibody

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产品名称: p70 S6 Kinase Antibody
产品型号:
产品展商: immunoclone
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简单介绍

p70 S6 Kinase Antibody迅速增长的产品线包括一抗、二抗、**测定及细胞检测试剂盒、蛋白/多肽、激动剂/拮抗剂/活化剂/抑制剂及裂解物等。p70 S6 Kinase Antibody严格的质量控制和验证实验包括各种不同的应用:WB、细胞**荧光、**组化、流式细胞术、ELISA、CHIP、IP和peptide array。


p70 S6 Kinase Antibody  的详细介绍

p70 S6 Kinase AntibodyProduct Profile

Product Name p70 S6 Kinase Antibody
Antibody Type Primary Antibodies
Product description
					
RPS6KB1 phosphorylates the Ribosomal Protein-S6. Activation of RPS6KB1 requires a complex, ordered series of conformational changes and phosphorylation reactions. While the role of sequential, multi-site phosphorylation has been extensively detailed, characterization of the priming step required to initiate this cascade has remained elusive. Probably this priming process is dependent on calcium. Calcium-dependent regulation of RPS6KB1 does not specifically target Thr-229 and Thr-389, the key regulatory phosphorylation sites; rather, calcium chelation results in a global inhibition of RPS6KB1 phosphorylation. The initial calcium-dependent process is required to release an inhibitory interaction between the C- and N-termini of RPS6KB1, thus allowing phosphorylation of key domains. The priming event involves formation of a calcium-dependent protein complex that releases the interaction between the N- and C-termini. RPS6KB1 is then accessible for activation by the kinases that target the known regulatory phosphorylation sites .1) Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851Papst PJ, et al. (1998) J Biol Chem. 273 (24): 15077-84. Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 cLe, X. F, et al. (2003) Oncogene 22: 484–97
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Immunogen
p70 S6 Kinase (Ab-424) antibody was raised against a peptide sequence around aa.422~426 (P-V-S-P-V) derived from Human p70S6k.

p70 S6 Kinase AntibodyKey Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IFIHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

p70 S6 Kinase AntibodyTarget Information

Gene Symbol RPS6KB1
Alternative Names
S6K
PS6K
S6K1
STK14A
p70-S6K
p70S6KA
p70-alpha
S6K-beta-1
p70(S6K)-alpha
PKR
PRKR
KS6B1
P70-S6K
RPS6KB1
S6K
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Molecular Weight(MW) 70, 85 kDa
Tissue Specificity This antibody detects endogenous level of total p70 S6 Kinase protein.

p70 S6 Kinase AntibodyDatabase Links

Entrez Gene 6198
Protein Accession NP_003152.1

p70 S6 Kinase AntibodyApplication

  • Application

    Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (Ab-424). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using p70 S6 Kinase (Ab-424).

  • Application

    Western blot analysis of lysed extracts from 293 cells using p70 S6 Kinase (Ab-424).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:

Additional Information

Form Liquid
Storage Instructions Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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