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Raf1 (phospho Ser259) Antibody

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产品名称: Raf1 (phospho Ser259) Antibody
产品型号:
产品展商: immunoclone
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简单介绍

Raf1 (phospho Ser259) Antibody迅速增长的产品线包括一抗、二抗、**测定及细胞检测试剂盒、蛋白/多肽、激动剂/拮抗剂/活化剂/抑制剂及裂解物等。Raf1 (phospho Ser259) Antibody严格的质量控制和验证实验包括各种不同的应用：WB、细胞**荧光、**组化、流式细胞术、ELISA、CHIP、IP和peptide array。


Raf1 (phospho Ser259) Antibody  的详细介绍

Raf1 (phospho Ser259) AntibodyProduct Profile

Product Name Raf1 (phospho Ser259) Antibody
Antibody Type Primary Antibodies
Product description 
					

						Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.1) Dougherty M K, et al. (2005) Mol Cell. 17 (2): 215-224. Hekman M, et al. (2005) FEBS Lett. 579 (2): 464-468. Avruch J, et al. (1994) Trends Biochem. Sci. 19, 279-283. Chong H, et al. (2001) EMBO J. 20, 3716-3727. King A J, et al. (1998) Nature. 396:180-183.
					

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Immunogen 
Raf1 (Phospho-Ser259) antibody was raised against a peptide sequence around phosphorylation site of serine 259 (S-T-S (p) -T-P) derived from Human Raf1.

Raf1 (phospho Ser259) AntibodyKey Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Raf1 (phospho Ser259) AntibodyTarget Information

Gene Symbol RAF1
Alternative Names
NS5
CRAF
Raf-1
c-Raf
BBC6
BCL2L8
C-RAF
C-Raf
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Molecular Weight(MW) 73 kDa
Tissue Specificity This antibody detects endogenous level of Raf-1 only when phosphorylated at serine 259.

Raf1 (phospho Ser259) AntibodyDatabase Links

Entrez Gene 5894
Protein Accession NP_002871.1

Raf1 (phospho Ser259) AntibodyApplication

  • Application

    Western blot analysis of lysed extracts from 293 and MDA cells untreated or treated with PMA using Raf1 (Phospho-Ser259).

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf1 (Phospho-Ser259).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
Storage Instructions Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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