Product Name	Mouse Map2k1 Antibody
Antibody Type	Primary Antibodies
Product description	Map2k1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.1) Chandrakesan, P., et al. J. Biol. Chem. 285(43):33485-33498(2010)
Immunogen	This Mouse Map2k1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-296 amino acids from the C-terminal region of mouse Map2k1.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
Tested Applications	IHC-PWB
	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:
Species Reactivity	HumanMouse
Concentration	1mg/ml
Purification	Affinity purified

Gene Symbol	Map2k1
Alternative Names	Dual specificity mitogen-activated protein kinase kinase 1 MAP kinase kinase 1 MAPKK 1 ERK activator kinase 1 MAPK/ERK kinase 1 MEK 1 Map2k1 Mek1 Prkmk1 More
Molecular Weight(MW)	43 kDa
Sequence Similarities	Predicted species reactivity based on immunogen sequence: Hamster

Entrez Gene	26395
Protein Accession	P31938

Application

Mouse Map2k1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application

Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).
Application

Western blot analysis of Map2k1 using rabbit polyclonal Mouse Map2k1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map2k1 gene.
Application

Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).

Application Notes	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:

Form	Liquid
Storage Instructions	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.