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NCI-H1105细胞

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产品名称: NCI-H1105细胞
产品型号: NCI-H1105
产品展商: HZbscience
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简单介绍

NCI-H1105细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。NCI-H1105细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。


NCI-H1105细胞  的详细介绍

NCI-H1105细胞

是否是肿瘤细胞: 1

物种来源: 人

细胞形态: 其他

生长状态: 悬浮生长

ATCC Number: CRL-5856™

数量: 大量

相关**: 小细胞肺癌

运输方式: 冻存运输

年限: stage E

器官来源: 肺

Designations: NCI-H1105 [H1105]

NCI-H1105细胞Depositors: AF Gazdar, JD Minna

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: floating clusters


Source: Organ: lung

NCI-H1105细胞Tumor Stage: stage E

Disease: carcinoma; classic small cell lung cancer

Derived from metastatic site: lymph node

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

Age: 73 years

Gender: male

Ethnicity: Caucasian

Comments: NCI-H1105细胞The line was established in July 1985.

The patient was a smoker.

50 pack years.

Propagation: ATCC complete growth medium: HITES medium supplemented with 5% fetal bovine serum

The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium

0.005 mg/ml Insulin

0.01 mg/ml Transferrin

30nM Sodium selenite (final conc.)

10 nM Hydrocortisone (final conc.)

10 nM beta-estradiol (final conc.)

extra 2mM L-glutamine (for final conc. of 4.5 mM)

5% fetal bovine serum (final conc.)


Temperature: 37.0°C

Preservation: Freeze medium: culture medium with 10% DMSO and extra 5% FBS

Storage temperature: NCI-H1105细胞liquid nitrogen vapor phase

Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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