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Homo sapiens细胞

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产品名称: Homo sapiens细胞
产品型号: Homo sapiens
产品展商: HZbscience
产品文档: 无相关文档

简单介绍

Homo sapiens细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。Homo sapiens细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。


Homo sapiens细胞  的详细介绍

Homo sapiens细胞

生长状态: 贴壁生长

数量: 大量

年限: Neonatal

运输方式: 冻存运输

ATCC Number: PCS-100-013

细胞形态: 其他

是否是肿瘤细胞: 0

物种来源: 人

Name: Primary Umbilical Vein Endothelial Cells; Normal, Human, Pooled

Biosafety Level: 1

Homo sapiens细胞Shipped: frozen

Organism: Homo sapiens (human)

Source: Umbilical vein endothelium

Growth Properties: Adherent

Age: Neonatal

Morphology: Cobblestone appearance

Volume per Vial: 1 ml

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Related Products: Vascular Cell Basal Medium (ATCC PCS-100-030)

Endothelial Cell Growth Kit/BBE (ATCC PCS-100-040)

Endothelial Cell Growth Kit/VEGF (ATCC PCS-100-041)

Homo sapiens细胞Dulbecco's Phosphate Buffered Saline (D-PBS) (ATCC 30-2200)

Trypsin-EDTA for Primary Cells (ATCC PCS-999-003)

Trypsin Neutralizing Solution (ATCC PCS-999-004)

Gentamicin-Amphotericin B Solution (ATCC PCS-000-025)

Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)

Phenol Red (ATCC PCS-999-001)

Recomended Culture Conditions: See Product Information Sheet

Comments: The cells are cryopreserved in the second passage to ensure the highest viability and plating efficiency. ATCC primary pooled HUVECs are derived from 10 individual donors, minimizing the lot-to-lot variability associated with cells derived from single donors. Large lot sizes assure consistency for large-scale studies.

A complete solution to propagate pooled HUVECs in low serum conditions with or without the addition of human recombinant VEGF. Use of the Endothelial Cell Growth Kit-VEGF will support a faster rate of proliferation because of the presence of several purified human recombinant (rh) growth factors (rh VEGF, rh EGF, rh FGF basic and rh IGF-1) combined with heparin and hydrocortisone. Use of the Endothelial Cell Growth Kit-BBE, which contains Bovine Brain Extract Homo sapiens细胞(BBE), is recommended if a more "authentic," less defined culture medium is desired.

Applications: Drug screening, angiogenesis, tubule formation, immune response, arteriolosclerosis, hypertension, vascular wound healing, inflammation, tissue remodeling, vascular differentiation, oxidative stress, apoptosis, stent development

Viability: ≥ 50%

Population Doubling Capacity: ≥ 15 in complete growth medium

Sterility Tests: Bacteria and Yeast: Negative

Mycoplasma: Negative

Testing: Hepatitis B: Negative

Hepatitis C: Negative

Mycoplasma: Negative

HIV-1 and HIV-2: Homo sapiens细胞Negative

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