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pCaspase3-sensor

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产品名称: pCaspase3-sensor
产品型号:
产品展商: HZbscience
产品文档: 无相关文档

简单介绍

pCaspase3-sensor的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到pCaspase3-sensor后请短暂离心,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。


pCaspase3-sensor  的详细介绍

pCaspase3-sensor载体基本信息

载体名称: pCaspase3-sensor
质粒类型: 信号通路报告系统载体
高拷贝/低拷贝: --
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4.8kb
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: EYFP
载体抗性: 卡纳霉素(Kanamycin)
筛选标记: 新霉素(Neomycin)
备注: --
产品目录号: 8185-1
稳定性: --
组成型: --
病毒/非病毒: --

pCaspase3-sensor载体质粒图谱和多克隆位点信息

pCaspase3-sensor



pCaspase3-sensor

pCaspase3-sensor载体简介

pCaspase3-Sensor Vector can be used to detect the onset of caspase-3 activity in mammalian cells.
This vector encodes the enhanced yellow-green variant (EYFP) of the Aequorea victoria green
fluorescent protein (GFP) fused at the 3' end to three copies of the nuclear localization signal (NLS)
of the simian virus 40 large T-antigen (1,2). At the 5' end the gene contains a sequence encoding
the nuclear export signal (NES) of the Map Kinase Kinase (MAPKK;3). The NES is separated from
the EYFP coding region by a 36-nucleotide sequence encoding the region of Poly (ADP-ribose)
polymerase (PARP) cleaved by caspase-3. The complete coding sequence for this fusion protein
is human codon-optimized (4).
Because the NES of MAPKK dominates the NLS, the full-length fluorescent fusion protein distributes
to the cytosol. If caspase-3 is activated, the NES will be cleaved from the fusion protein and the
truncated EYFP-NLS fusion will translocate to the nucleus via the NLS. The translocation of the
fluorescent protein from the cytosol to the nucleus indicates caspase-3 activity at a cellular level.
The fluorescence excitation maximum of EYFP is 513 nm; the emission spectrum has a peak at 527
nm (in the yellow-green region). The Em of EYFP at 513 nm is 36,500 cm–1M–1 and its quantum yield
is 0.63 (5), resulting in a bright fluorescent signal. The fluorescence intensity is roughly equivalent
to that of EGFP.
The vector contains an SV40 origin of replication and a neomycin resistance (Neor) gene for
selection (using G418) in eukaryotic cells. A bacterial promoter (P) upstream of Neor expresses
kanamycin resistance in E. coli. The vector backbone also provides a pUC19 origin of replication for
propagation in E. coli and an f1 origin for single-stranded DNA production. The pCaspase3-Sensor
Vector can be introduced into mammalian cells using any standard transfection method. If desired,
stable transfectants can be selected using G418 (6). 

pCaspase3-sensor载体序列

hz-7021R IFNA21/IFNA F  干扰素α21抗体
hz-7022R IFNAR2/IFNABR  干扰素α受体2抗体
hz-7023R IFNA11/Limitin/Interferon alpha 11  干扰素α11抗体
hz-7024R IFNA10/Interferon alpha10/Interferon alpha C  干扰素α10抗体
hz-7025R Otoraplin/OTOR/Fibrocyte derived protein  纤维细胞源性蛋白
hz-7026R HSP10/CPN10  热休克蛋白10抗体
hz-7027R ANGPTL6  血管生成素相关蛋白6抗体
hz-7028R RCAN2/DSCR1L1  钙调神经磷酸酶调节蛋白2抗体(唐氏综合征候选区域蛋白1样蛋白1)
hz-7029R EDIL3  表皮生长因子样重复折叠1结构域蛋白3抗体
hz-7030R GLMN/FK506 binding protein associated protein  FK506结合蛋白相关蛋白抗体(他克莫司相关蛋白)
hz-1416R Aryl Hydrocarbon Receptor/AHR  芳香烃受体抗体
hz-7031R MEOX 2  间充质同源盒蛋白2抗体
hz-7032R EphA3/Eph receptor A3  酪氨酸蛋白激酶受体A3抗体
hz-7033R EphA6/Eph receptor A6  酪氨酸蛋白激酶受体A6抗体
hz-7034R EphA7/Eph receptor A7  酪氨酸蛋白激酶受体A7抗体
hz-7035R EphA8/Eph receptor A8  酪氨酸蛋白激酶受体A8抗体
hz-7037R EphB1/Eph receptor B1  酪氨酸蛋白激酶受体B1抗体
hz-7038R phospho-EphB1/Eph receptor B1(Tyr317)  磷酸化酪氨酸蛋白激酶受体B1抗体
hz-7039R phospho-EphB1/Eph receptor B1(Tyr331)  磷酸化酪氨酸蛋白激酶受体B1抗体

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