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E2F1 antibody

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产品名称: E2F1 antibody
产品型号:
产品展商: immunoclone
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简单介绍

E2F1 antibody迅速增长的产品线包括一抗、二抗、**测定及细胞检测试剂盒、蛋白/多肽、激动剂/拮抗剂/活化剂/抑制剂及裂解物等。E2F1 antibody严格的质量控制和验证实验包括各种不同的应用：WB、细胞**荧光、**组化、流式细胞术、ELISA、CHIP、IP和peptide array。


E2F1 antibody  的详细介绍

E2F1 antibodyProduct Profile

Product Name Rabbit anti-E2F1 polyclonal antibody - middle region
Antibody Type Primary Antibodies

E2F1 antibodyKey Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WB

Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

E2F1 antibodyTarget Information

Gene Symbol E2F1
Gene Synonyms
E2F-1
RBAP1
RBBP3
RBP3
More 
Gene Full Name E2F transcription factor 1
Gene Summary
The protein encoded by E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
More 
Alternative Names
E2F-1
RBAP1
RBBP3
RBP3
More 
Molecular Weight(MW) 47kDa
Sequence 437 amino acids

E2F1 antibodyDatabase Links

Entrez Gene 1869
SwissProt ID Q01094
Protein Accession NP_005216

E2F1 antibodyApplication

  • Western blot

    Titration: 1.0 ug/ml
    Positive Control: Fetal Lung

  • Immunoblot

    1. 30 ug Cytosol extract: Untransfected cells 2. 30 ug Cytosol extract: Cells transfected with control (non-targeting) siRNA 3. 30 ug Cytosol extract: Cells transfected with anti-E2F1-siRNA 4. 30 ug Cytosol extract: Cells transfected with anti-E2F1-siRNA
    Primary Antibody Dilution: 
    1:2000
    Secondary Antibody: 
    Goat anti-rabbit HRP
    Secondary Antibody Dilution: 
    1:2000
    Gene Name: 
    E2F1
    Submitted by: 
    Ewelina Swiderek, Insitute of Immunology and Experimental Therapy, Wroclaw, Poland

Additional Information

Form Liquid
Storage Instructions Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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