物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
来源单抗制备
宿主小鼠
效价(克隆号)C11
Ig亚型IgG2b Kappa
纯化方式蛋白A + 蛋白G亲和纯化
标记物无标记物
**原RPC472Mu01-衰老关键蛋白1(FBLN1)重组蛋白
缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
性状液体
浓度500ug/ml
且适物种-
应用WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM
衰老关键蛋白1(FBLN1)单克隆抗体 Monoclonal Antibody to Fibulin 1 (FBLN1)
Organism Species: Mus musculus (Mouse)
Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 11th Edition (Revised in May, 2016)
[ PROPERTIES ] Source: Monoclonal antibody preparation Host: Mouse Antibody isotype: IgG2b Kappa Purification: Protein A/G Affinity Chromatography. Clone number: C11 Traits: Liquid Concentration: 500µg/mL UOM: 200µg Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
衰老关键蛋白1(FBLN1)单克隆抗体[ IMMUNOGEN ] Immunogen: Recombinant FBLN1 (Cys399~Arg578) expressed in E.coli. Accession No.: RPC472Mu01
[ APPLICATIONS ] Western blotting: 0.5-5ug/ml Immunocytochemistry in formalin fixed cells: 5-30ug/ml Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml Immunohistochemistry in paraffin section: 5-30ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user.
衰老关键蛋白1(FBLN1)单克隆抗体 [ FORMULATION ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ] Content: The quality control contains recombinant FBLN1 disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine (DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3.
[ STORAGE AND STABILITY ] Storage: Avoid repeated freeze/thaw cycles. Store at 4 oC for frequent use. Aliquot and store at -20 oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 oC for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
衰老关键蛋白1(FBLN1)单克隆抗体[ IDENTIFICATION ] Figure 1. Western Blot A. Sample: Recombinant FBLN1, Mouse A