来源单抗制备
宿主小鼠
效价(克隆号)-
Ig亚型IgG
纯化方式抗原特异性亲和纯化
肝细胞生长因子(HGF)单克隆抗体标记物无标记物
**原-
缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
性状液体
浓度500ug/ml
且适物种-
应用WB, ICC, IHC-P, IHC-F, ELISA
肝细胞生长因子(HGF)单克隆抗体Monoclonal Antibody to Hepatocyte Growth Factor (HGF)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: HGF, Human
Clonality: Monoclonal
Clone number: B2
Host: Mouse
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 500µg/mL
UOM: 200µg Sample: Recombinant HGF, Human
肝细胞生长因子(HGF)单克隆抗体[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant HGF (Val495~Ser728) expressed in E.coli. Accession No.: RPA047Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and
T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS - VVNGIP TRTNIGWMVS
LRYRNKHICG GSLIKESWVL TARQCFPSRD LKDYEAWLGI HDVHGRGDEK CKQVLNVSQL
VYGPEGSDLV LMKLARPAVL DDFVSTIDLP NYGCTIPEKT SCSVYGWGYT GLINYDGLLR
VA H LYIM G NE K CS Q H H R G K V TL N E S EI C A G AE K I GS G P CE GDY G G P LV C E
QHKMRMVLGV IVPGRGCAIP NRPGIFVRVA YYAKWIHKII LTYKVPQS
[ ANTIBODY SPECIFITY ]
The antibody is a mouse monoclonal antibody raised against HGF. It has been
selected for its ability to recognize HGF in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
肝细胞生长因子(HGF)单克隆抗体[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.