来源单抗制备
宿主小鼠
效价(克隆号)-
Ig亚型IgG
纯化方式抗原特异性亲和纯化
碳酸酐酶Ⅳ(CA4)单克隆抗体标记物无标记物
**原-
缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
性状液体
浓度500ug/ml
且适物种-
应用WB, ICC, IHC-P, IHC-F, ELISA
碳酸酐酶Ⅳ(CA4)单克隆抗体Monoclonal Antibody to Carbonic Anhydrase IV (CA4)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: CA4, Human
Clonality: Monoclonal
Clone number: D6
Host: Mouse
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 200µg Sample: Recombinant CA4, Human
碳酸酐酶Ⅳ(CA4)单克隆抗体[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant CA4 (Ala19~Lys283) expressed in E.coli. Accession No.: RPD071Hu01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence
is listed below.
MGHHHHHHSGSEF-AE SHWCYEVQAE SSNYPCLVPV KWGGNCQKDR QSPINIVTTK
AKV DKK LG RF FFSGY DKK QT WTVQ NNG HSV MM LLE NKASI SG GG LPAPYQ
AKQLHLHWSD LPYKGSEHSL DGEHFAMEMH IVHEKEKGTS RNVKEAQDPE DEIAVLAFLV
EAGTQVNEGF QPLVEALSNI PKPEMSTTMA ESSLLDLLPK EEKLRHYFRY LGSLTTPTCD
EKVVWTVFRE PIQLHREQIL AFSQKLYYDK EQTVSMKDNV RPLQQLGQRT VIK
[ ANTIBODY SPECIFITY ]
The antibody is a mouse monoclonal antibody raised against CA4. It has been
selected for its ability to recognize CA4 in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
碳酸酐酶Ⅳ(CA4)单克隆抗体[ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.