来源单抗制备
宿主小鼠
效价(克隆号)-
Ig亚型IgG
纯化方式抗原特异性亲和纯化
晚期糖基化终末产物(AGE)单克隆抗体标记物无标记物
**原-
缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
性状液体
浓度500ug/ml
且适物种-
应用WB, ICC, IHC-P, IHC-F, ELISA
晚期糖基化终末产物(AGE)单克隆抗体Monoclonal Antibody to Advanced Glycation End Product (AGE)
Organism Species: General
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: AGE
Clonality: Monoclonal
Clone number: C5
Host: Mouse
Immunoglobulin Type: IgG1 Kappa
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 500µg/mL
UOM: 200µg
晚期糖基化终末产物(AGE)单克隆抗体[ IMMUNOGEN INFORMATION ]
Immunogen: Protein AGE
Accession No.: NPB353Ge01
[ RELEVANCE ]
An advanced glycation end-product (AGE) is the result of a chain of chemical
reactions after an initial glycation reaction. Glycation is accomplished by the
Maillard reaction, which is a multistep process that begins with Schiff base
formation between the amine and the carbonyl group on the sugar followed by
rearrangement to form Amadori intermediates. AGEs affect nearly every type of
cell and molecule in the body, and are thought to be one factor in aging and some
age-related chronic diseases. BSA is glycosylated by the reaction of the glucose
and BSA in vitro, and then being multistep purification, ending in high puified
AGEs.
[ ANTIBODY SPECIFITY ]
The antibody is a mouse monoclonal antibody raised against AGE. It has been
selected for its ability to recognize AGE in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
晚期糖基化终末产物(AGE)单克隆抗体[ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.