来源单抗制备
宿主小鼠
效价(克隆号)D4
Ig亚型IgG2a Kappa
纯化方式蛋白A + 蛋白G亲和纯化
肿瘤坏死因子α(TNFa)单克隆抗体标记物无标记物
缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
性状液体
浓度500ug/ml
且适物种-
应用WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
肿瘤坏死因子α(TNFa)单克隆抗体Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Monoclonal antibody preparation
Host: Mouse
Antibody isotype: IgG2a Kappa
Purification: Protein A/G Affinity Chromatography. Clone number: D4
Traits: Liquid
Concentration: 500µg/mL
UOM: 200µg
Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ]
Immunogen: Recombinant TNFa (Val77~Leu233) expressed in E.coli. Accession No.: RPA133Hu01
[ APPLICATIONS ]
Western blotting: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user. [ FORMULATION ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,
50% glycerol.肿瘤坏死因子α(TNFa)单克隆抗体 [ QUALITY CONTROL ]
Content: The quality control contains recombinant TNFa disposed in loading
buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine (DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 4
oC for frequent use. Aliquot and store at -20
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition.肿瘤坏死因子α(TNFa)单克隆抗体 [ IDENTIFICATION ]
A
Figure 1. Western Blot
A. Sample: Recombinant TNFa, Human