来源单抗制备
宿主小鼠
效价(克隆号)1:270000 (8#)
Ig亚型IgG
纯化方式抗原特异性亲和纯化
转化生长因子α(TGFα)单克隆抗体标记物无标记物
**原-
缓冲液成份磷酸盐缓冲液（pH7.4, 含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
性状液体
浓度500ug/ml
且适物种-
应用WB, ICC, IHC-P, IHC-F, ELISA
转化生长因子α(TGFα)单克隆抗体Monoclonal Antibody to Transforming Growth Factor Alpha (TGFa)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PRODUCT INFORMATION ]
Immunogen: TGFa, Human
Clonality: Monoclonal
Clone number: C6
Host: Mouse
Immunoglobulin Type: IgG1 Kappa
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 500µg/mL
UOM: 200µg
转化生长因子α(TGFα)单克隆抗体[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant TGFa (Glu24~Ala98) with two N-terminal Tags,
His-tag and T7-tag expressed in E.coli. Accession No.: RPA123Hu01
[ ANTIBODY SPECIFITY ]
The antibody is a mouse monoclonal antibody raised against TGFa. It has been
selected for its ability to recognize TGFa in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant TGFa (Glu24~Ala98)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
转化生长因子α(TGFα)单克隆抗体[ IMAGES ]
Used in Western Blot, Sample:
Recombinant TGFa, Human