来源单抗制备
宿主小鼠
效价(克隆号)C3
Ig亚型IgG2a Kappa
纯化方式蛋白A + 蛋白G亲和纯化
转化生长因子β1(TGFb1)单克隆抗体标记物无标记物
缓冲液成份磷酸盐缓冲液（pH7.4, 含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
性状液体
浓度500ug/ml
且适物种-
应用WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
转化生长因子β1(TGFb1)单克隆抗体Monoclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
12th Edition (Revised in Aug, 2016)
[ PROPERTIES ]
Source: Monoclonal antibody preparation
Host: Mouse
Antibody isotype: IgG2a Kappa
Purification: Protein A/G Affinity Chromatography. Clone number: C3
Traits: Liquid
Concentration: 2mg/mL
UOM: 200µg
Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ]
Immunogen: Recombinant TGFb1 (Ala279~Ser390) expressed in E.coli. Accession No.: RPA124Hu01
转化生长因子β1(TGFb1)单克隆抗体[ APPLICATIONS ]
Western blotting: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user. [ FORMULATION ]
Form & Buffer: Supplied as solution form in 0.01M PBS, pH7.4, containing
0.05% Proclin-300, 50% glycerol. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 4
oC for frequent use. Aliquot and store at -20
oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed. The loss rate is less than 5% within the expiration date under
appropriate storage condition. 转化生长因子β1(TGFb1)单克隆抗体[ IDENTIFICATION ]
Figure 1. Western Blot
Sample: Recombinant TGFb1, Human